BIOM-50. THE IDENTIFICATION AND FUNCTIONAL CHARACTERIZATION OF CIRCULAR RNA CIRC_63706 IN SONIC HEDGEHOG MEDULLOBLASTOMAS

Abstract Medulloblastoma is a central nervous system tumor that develops through various genetic, epigenetic, and non-coding (nc) RNA-related mechanisms, but the roles played by ncRNAs, particularly circular RNAs (circRNAs), remain poorly defined. CircRNAs are increasingly recognized as stable noncoding RNA therapeutic targets in many cancers, little known about their function, subtype specificity, potential medulloblastomas. To determine medulloblastoma subgroup-specific circRNAs, we subjected RNA-seq data from 175 clinical samples four subgroups (SSH, WNT, G3, G4) to statistical machine learning (random forest) pipeline identified group of specific RNAs. CircRNA, circ_63706 was sonic hedgehog (SHH) confirmed its expression RNA-FISH analysis tissue (tissue microarrays). identify molecular function circ_63706, depleted DAOY ONS76 cells them global sequencing lipid profiling. Circ_63706 resides coding gene Pericentrin (PCNT), which be involved congenital disorders. When gets shRNA, it shows significant decrease cell proliferation invasion SSH cells, mice implanted with circ_63706-deleted showed reduced growth extended survival compared parental implant. At level, elevated total ceramide oxidized lipids triglyceride (TG). Our study implicates an identification novel oncogenic subgroup establishes future target.